![]() Dose–response curves were established from the optical signal of the immunocomplex and unbound AuNP-Ab conjugate fractions. ![]() The readout was executed either in a direct or in indirect way by measuring the UV–Visible spectrum of each fraction in a specially designed microcell. Addition of the target analyte followed by detection probe resulted in the formation of a sandwich immunocomplex which was separated from the unbound AuNP-Ab conjugate by application of an external magnetic field. The AuNP bioconjugate was used as the optical detection probe while the MB one was used as the capture probe. Both spherical gold nanoparticles (AuNP) and magnetic beads (MB) were conjugated to goat anti-rabbit IgG antibody (Ab) capable of recognizing a model target, rabbit IgG (rIgG). We designed a magneto-plasmonic biosensor for the immunodetection of antigens in minute sample volume.
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